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INVITED PRESENTATIONS
 

P. S. Tsai, QBio: An Integrative Educational Program of Physics and Biology at UCSD, AAPT Winter Meeting (Jan, 2018)

P. S. Tsai, Guiding the Transition: Biophysics Laboratory on Research Design and Implementation.  AAPT Winter meeting (Jan, 2015).

P. S. Tsai, Optical tools for high-resolution, large-field measurements in the brain.  Optics and photonics research at UCSD: materials, metamaterials, living and synthetic matter (Aug, 2014).

P. S. Tsai, Mouse cerebral blood flow and reconstruction of the cortical angioarchitecture. VRC Seminar at Katholieke Universiteit Leuven  (Apr, 2014).

P. S. Tsai, Blood flow and the angioarchitecture of mouse cerebral cortex . Neuronus 2014 (Apr, 2014).

P. S. Tsai, Bloodflow and the angio-architecture in relation to the vibrissa barrels. Barrels XXVI (Nov, 2013).

P. S. Tsai, D. C. Jeong, D. Kleinfeld,  Precise Cranial Surgery With Femtosecond Laser Ablation, Laser Induced Breakdown Spectroscopy and Second Harmonic Generation.  SCIX / North American Society for Laser Induced Breakdown Spectroscopy (Oct, 2013).

P. S. Tsai, P. Blinder, J. P. Kaufhold, and D. Kleinfeld, From Photons to Flow Patterns – Quantifying neurovascular architecture with light microscopy. Janelia Farms Conference on Multiphoton Imaging: The Next 6x10^23 Femtoseconds (April, 2011)

P. Blinder, P. S. Tsai, J.P. Kaufhold, and D. Kleinfeld, Reconstruction of the cortical vascular network in mouse, Computational Neurosciences Meeting, Workshop on High-throughput 3D microscopy and high-performance computing for multi-scale modeling and simulation of large-scale neuronal circuits (July, 2010)

P. S. Tsai, P. Blinder, P. Drew, J. Driscoll, D. Jeong, J.P. Kaufhold, A. Shih, I. Valmianski, and D. Kleinfeld, Topology, dynamics, and control in cortical blood flow elucidated with optical techniques, SPIE Optics and Photonics (August, 2009)

P. S. Tsai, P. Blinder, B. Migliori, D. Kleinfeld, Ultra-Short Laser Pulses as a Tool to Measure as well as Perturb Neurovascular Activity in the Rodent Brain, Optical Society of America, Annual Meeting: Frontier in Optics/ Laser Science (October, 2008)

P. S. Tsai, Challenges in the analysis and acquisition of 3-D in vivo imaging data, Fourth International Workshop Statistical Analysis of Neuronal Data (May, 2008)

P. S. Tsai, B. Friedman, A. I. Ifarraguerri, B. D. Thompson, V. Lev-Ram, C. B. Schaffer, Q. Xiong, R. Y. Tsien, J. A. Squier, and D. Kleinfeld, All optical histology: Serial ablation and Multiphoton Imaging of Neuronal Tissue with Femtosecond Laser Pulses, IEEE Lasers and Electro Optics Society Annual Meeting (October, 2003)

P. S. Tsai, B. Friedman, V. Lev-Ram, Q. Xiong, R. Y. Tsien, D. Kleinfeld, A. I. Ifarraguerri, B. D. Thompson, and J. A. Squier*, All optical histology of brain tissue: Serial ablation and multiphoton imaging with femtosecond laser pulses, Conference on Lasers and Electro-Optics (May, 2003)




CONTRIBUTED PRESENTATIONS
 

P. Blinder, P. S. Tsai, J. P. Kaufhold, P. M. Knutsen, H. Suhl and D. Kleinfeld, Highly interconnected microvasculature supports restricted blood flow independent of columnar boundaries, 21st Annual Meeting of the Israel-Society-for-Neuroscience (Nov, 2013).

P. S. Tsai, M. Anderson,  J. Field, C. Mateo, and D. Kleinfeld, Ultrawide-field two photon laser scanning microscope, Society for Neuroscience Annual Meeting (November, 2013).

C. Mateo, P. S. Tsai, A. Y. Shih, and D. Kleinfeld,  Probing neurovascular coupling via natural and driven vasomotion in the awake mouse, Society for Neuroscience Annual Meeting (November, 2013).

P. S. Tsai, P. Blinder, J. Kaufhold, B. Friedman, P. D. Lyden, and D. Kleinfeld, The murine angiotome: Patterns of blood flow in mouse vibrissa sensory cortex revealed by modeling flow in vectorized reconstructions of the vasculature, Society for Neuroscience Annual Meeting (November, 2011)

A. Y. Shih, P. Blinder, P. S. Tsai, G. M. Stanley, B. Friedman, P. D. Lyden, and D. Kleinfeld, The smallest stroke: Occlusion of a single penetrating vessel in cortex leads to local pathology and sensory impairment, Society for Neuroscience Annual Meeting (November, 2011)

P. Blinder, P. S. Tsai, J. P. Kaufhold, and D. Kleinfeld, Topological basis for differences in surface versus subsurface blood flow from the vectorization of all vessels and cell nuclei across cortical columns, Society for Neuroscience Annual Meeting (November, 2010)

P .S. Tsai, P. J. Drew, A. Y. Shih, P. Knutsen, D. Davalos, P. Blinder, K. Akassaglou, and D. Kleinfeld, Large-scale chronic imaging and optical manipulation of cortical cells through a polished and reinforced thinned-skull, Society for Neuroscience Annual Meeting (November, 2010)

D. Jeong, P. S. Tsai, D. Schafer, J. Squier, Y. Neev, and D. Kleinfeld, All optical cranial surgery: Ablation of hard but not soft tissue by amplified femtosecond laser pulses and feedback from atomic emission spectra, Society for Neuroscience Annual Meeting (November, 2010)

P. S. Tsai, P. Blinder, J. P. Kaufhold, B. Friedman, P. D. Lyden, and D. Kleinfeld, Vascular and cellular maps of mouse cortex revealed through all-optical histology and volumetric image data analysis algorithms, Society for Neuroscience Annual Meeting (November, 2009)

P. J. Drew, J. D. Driscoll, P. S. Tsai, A. Y. Shih, and D. Kleinfeld, Two-photon imaging of spontaneous and sensory evoked cortical microvascular dynamics in the awake mouse, Society for Neuroscience Annual Meeting (November, 2009)

J. Kaufhold, P. S. Tsai, P. Blinder and D. Kleinfeld. Threshold relaxation is an effective means to connect gaps in 3D images of complex microvascular networks, Microscopic Image Analysis with Applications in Biology / Medical Image Computing and Computer Assisted Intervention (September, 2008)

P. Blinder, P. S. Tsai, J. Kaufhold, A. Y. Shih, C. A. Rafie, B. Friedman, P. D. Lyden, and D. Kleinfeld, Towards a multi-scale complete mapping of the cortical rodent angioarchitecture and neuronal cell nuclei distribution: a potential structural basis for post-ischemic cell damage patterm. Gordon Conference on Brain Energy Metabolism & Blood Flow (August 2008),

P. S. Tsai, J. Kaufhold, B. Friedman, P. Lyden, D. Kleinfeld , Direct Counts of Cellular and Vascular Densities in Thick Cortical Sections of Mouse. , Society for Neuroscience Annual Meeting (November, 2007).

J. Kaufhold, P. S. Tsai, P. Binder, B. Friedman, P. Lyden, D. Kleinfeld, A. I. Ifarraguerri , Automatic Neuroanatomical Microvessel Segmentation, Nuclei Labeling and Diffusion Distance Computations with All Optical Histology, Society for Neuroscience Annual Meeting (November, 2007).

P. Binder, P. S. Tsai, J. Kaufhold, B. Friedman, D. Kleinfeld, Complete Millimeter-Scale Reconstruction of Cells and Vasculature in Mouse Barrel Cortex , Society for Neuroscience Annual Meeting (November, 2007).

B. Migliori, P. S. Tsai, K. Campbell, T. Kim, T. Kam, A. Groisman, D. Kleinfeld , A Flexible Polymer Lens Reduces Spherical Aberration in Two-Photon Microscopy, Society for Neuroscience Annual Meeting (November, 2007).

P. S. Tsai, T.N. Kim, K. Campbell, A. Groisman, Z. Kam, and D. Kleinfeld, Spherical aberration correction in deep multi-photon imaging, Optical Society of America, FiO (2005)

N. Nishimura, C. B. Schaffer, B. Friedman, P. S. Tsai, P. D. Lyden, and D. Kleinfeld, Collateral blood flow after occlusion of individual surface and subsurface microvessels, Barrels XVII (2004).

P. S. Tsai, B. Friedman, A. I. Ifarraguerri, C. B. Schaffer, V. Lev-Ram, R. Y. Tsien, and D. Kleinfeld, All-optical histology for the large-scale mapping of cellular and vasculature distributions in rodent brain, Focus on Microscopy Conference (April 2004).

N. Nishimura, C. B. Schaffer, B. Friedman, P. S. Tsai, P. D. Lyden, and D. Kleinfeld, Targeted microvascular disruption in rat cortex using ultrashort laser pulses, Focus on Microscopy (2004).

N. Nishimura, C. B. Schaffer, B. Friedman, P. S. Tsai*, P. D. Lyden, and D. Kleinfeld, Targeted disruption of deep-lying neocortical microvessels in rat using ultrashort laser pulses, Photonics West (2004).

A. Ifarraguerri, B. Thompson, P. S. Tsai, B. Friedman, C. B. Schaffer, V. Lev-Ram, R. Y. Tsien , J. A. Squier, and D. Kleinfeld, Quantitative analysis of volumetric TPLSM data, IEEE International Symposium on Biomedical Imaging (2004)

C. B. Schaffer, N. Nishimura, B. Friedman, P. S. Tsai, L. F. Schroeder, F. F. Ebner, P. D. Lyden, and D. Kleinfeld, Two-photon microscopy reveals that cortical blood flow reverses direction at the first branch that lies downstream from a localized photothrombotic clot, OSA Conference on Lasers and Electro-Optics (May, 2004)

P. S. Tsai, B. Friedman, A. I. Ifarraguerri, B. D. Thompson, V. Lev-Ram, C. B. Schaffer, Q. Xiong, R. Y. Tsien, J. A. Squier, and D. Kleinfeld , All optical histology of neuronal tissue using ultrashort pulses, Society for Neuroscience Annual Meeting (November, 2003).

N. Nishimura, C. B. Schaffer, P. S. Tsai, B. Friedman, P. D. Lyden, and D. Kleinfeld, Photodisruption with amplified femtosecond laser pulses induces three-dimensionally localized clots in neocortical vasculature in rat, Society for Neuroscience Annual Meeting (November, 2003)

C. B. Schaffer, F. F. Ebner, N. Nishimura, B. Friedman, P. S. Tsai, L. F. Schroeder, P. D. Lyden, and D. Kleinfeld, Two-photon fluorescence microscopy of collateral blood flow following photothrombotic stroke in rat neocortex, OSA Annual Meeting (Oct, 2003).

C. B. Schaffer, F. F. Ebner, N. Nishimura, B. Friedman, P. S. Tsai, L. F. Schroeder, P. D. Lyden, and D. Kleinfeld, Arteriole blood flow reverses direction at the first branch that lies downstream from a localized photothrombotic clot, Lasers and Electro Optics Society Annual Meeting (Oct, 2003)

P. S. Tsai, B. Friedman, V. Lev-Ram, Q. Xiong, R. Y. Tsien, D. Kleinfeld, A. I. Ifarraguerri, B. D. Thompson, and J. A. Squier*, All optical histology of brain tissue: Serial ablation and multiphoton imaging with femtosecond laser pulses, Conference on Lasers and Electro-Optics (May, 2003).

C. B. Schaffer, F. F. Ebner, N. Nishimura, B. Friedman, P. S. Tsai, L. F. Schroeder, P. D. Lyden, and D. Kleinfeld, Real-time two-photon fluorescence microscopy of blood flow dynamics following photothrombotic stroke in rat neocortex, Lasers and Electro-Optics Society, (May, 2003).

P. S. Tsai, B. Friedman, A. I. Ifarraguerri, B. D. Thompson, V. Lev-Ram, C. B. Schaffer, Q. Xiong, R. Y. Tsien, J. A. Squier, and D. Kleinfeld, All Optical histology of neuronal tissue : serial ablation and multiphoton imaging with femtosecond laser pulses, SPIE Photonics West (January 2003).

C. B. Schaffer, L. F. Schroeder, P. D. Lyden, N. Nishimura*, P. S. Tsai*, and D. Kleinfeld, Femtosencond laser-induced hemorrhagic and thrombolytic microstrokes in rodents, SPIE Photonics West (January 2003).

P. S. Tsai, J. A. Squier, and D. Kleinfeld, Precision, Large-Scale Cutting Of Neuronal Tissue with Femtosecond Laser Light, Biophysical Society Annual Meeting (Feburary 2002).

O. Clay, P. S. Tsai*, A. C. Millard, J. A. Squier, and D. Kleinfeld, Evidence for Resonances in the Third Harmonic Spectra of Hemoglobin as a Function of its Ligand Binding State, Biophysical Society Annual Meeting (Feburary, 2002).

O. Griesbeck, E. J. Yoder*, P. S. Tsai*, R. Y. Tsien, and D. Kleinfeld, In-Vivo Expression of Cameleons in Rodent Neocortex by Adenoviral Gene Transfer, Society for Neuroscience Annual Meeting (November, 2000).