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REFEREED PUBLICATIONS

32. A.F. Smith, V. Doyeux, M. Berg, M. Pyrounette, M. Haft-Javaherian, A.E. Larue, J.H. Slater, F. Lauwers, P. Blinder, P. S. Tsai, D. Kleinfeld, C.B. Schaffer, N. Nishimura,
         Y. Davit, and S. Lorthois, Brain Capillary Networks Across Species: A few Simple Organizational Requirements Are Sufficient to Reproduce Both Structure and       
         Function. Frontiers in Physiology (2019) 10:3389.

31. M. G. Baez-Yanez, P. Ehses, P. Mirkes, P. S. Tsai, D. Kleinfeld and K. Scheffler, The impact of vessel size, orientation and intravascular contribution on the
          neurovascular fingerprint of BOLD bSSFP fMRI. NeuroImage (2017) 163:13-23

30.  C. I. Mateo, P. M. Knutsen, P. S. Tsai, A. Y. Shih and D. Kleinfeld, Entrainment of arteriole vasomotor fluctuations by neuronal activity: A basis of blood oxygen level
          dependent "resting state" connectivity across cortex. Neuron (2017) 20(3):406-416

29.  K.Kisler, A. R. Nelson, S. V. Rege, A. Ramanathan, Y. Wang, A. Ahuja, D. Lazic, P. S. Tsai, Z. Zhao, Y. Zhou, D. A. Boas, S. Sakadzic & B. V. Zlokovic, Pericyte
          degeneration leads to neurovascular uncoupling and limits oxygen supply to brain. Nature Neuroscience (2017)

28.  F. Schmid, P. S. Tsai, D. Kleinfeld, P. Jenny and B. Weber, Depth dependent flow and pressure characteristics in cortical microvascular networks. Public Library of
           Science: Computational Biology (2017) 13:e1005392.

27.  I. Gould, P. S. Tsai, D. Kleinfeld and A. Linninger, The capillary bed offers the largest hemodynamic resistance to the cortical blood supply. Journal of Cerebral Blood
          Flow & Metabolism (2017) 37:52-68.

26.  P. S. Tsai, C. Mateo, J.J. Field, C.B. Schaffer, M.E. Anderson & D. Kleinfeld, Ultra-large field-of-view two-photon microscopy. Optics Express (2015) 23: 13833-13847

25.  A.Y. Shih, C. Ruehlmann, P. Blinder, A. Devor, P.J. Drew, B. Friedman, P.M. Knutsen, P.D. Lyden, C. Mateo, L. Mellander, N. Nishimura, C.B. Schaffer, P. S. Tsai and  
          D. Kleinfeld, Robust
          and Fragile Aspects of Cortical Blood Flow in Relation to the Underlying Angioarchitecture. Microcirculation (2015) 22:204-218

24.  D. C. Jeong, P. S. Tsai and D. Kleinfeld, All-optical osteotomy to create windows for transcranial imaging in mice. Optics
Express 21:23160-23168 (2013).

23.  P. Blinder*, P. S. Tsai*, J. P. Kaufhold, P. M. Knutsen, H. Suhl and D. Kleinfeld, The cortical angiome: An interconnected vascular network with noncolumnar
          patterns of blood flow. Nature Neuroscience 16:889-897 (2013).


22.  A. Y. Shih, P. Blinder, P. S. Tsai, B. Friedman, G. Stanley, P. D. Lyden and D. Kleinfeld,The smallest stroke: Occlusion of one penetrating vessel leads to
          infarction and a cognitive deficit. Nature Neuroscience  16:55-63 (2013).


21.  J. P. Kaufhold, P. S. Tsai, P. Blinder and D. Kleinfeld, Vectorization of optically sectioned brain microvasculature: Learning aids completion of vascular graphs
          by
connecting gaps and deleting open-ended segments, Medical Image Analysis (2012).

20.  A. Y. Shih, P. J. Drew, C. Mateo,
P. S. Tsai and D. Kleinfeld, A polished and reinforced thinned skull window for long-term imaging and optical manipulation of
          the mouse cortex,. Journal of Visualized Neuroscience, http://www.jove.com/video/3742, (2011).

19.  D.C. Jeong,
P. S. Tsai and D. Kleinfeld, Prospect for feedback guided surgery with ultra-short pulsed laser light, Current Opinions in Neurobiology, 22:24-33,
         
(2011).

18.  D. Kleinfeld, A. Bharioke, P. Blinder, D. D. Bock, K. L. Briggman, D. B. Chklovskii, W. Denk, M. Helmstaedter, J. P. Kaufhold, W-C. A. Lee, H. S. Meyer, K. D.
         
Micheva, M. Oberlaender, S. Prohaska, R. C. Reid, S. J. Smith, S. Takemura,
P. S. Tsai and B. Sakmann, Large-scale automated histology in the pursuit of
         
connectomes.
Journal of Neuroscience 31:16125-16138, (2011).

17.  D. Kleinfeld, P. Blinder, P. J. Drew, J. D. Driscoll, A. Muller, P. S. Tsai and A. Y. Shih, A guide to delineate the logic of neurovascular signaling in the brain.
         
Frontiers in Neuroenergetics, 3:1-9 (2010).

16.  P. J. Drew, A. Y. Shih, P. Knutsen, D. Davalos, P. Blinder, K. Akassoglou,  P. S. Tsai and D. Kleinfeld, Chronic imaging and optical manipulation of cortical cells
         
and microvessels through a large-area polished and reinforced thinned-skull.
Nature Methods.,981-984, (2010).

15.  D. N. Vitek, D. E. Adams, A. Johnson,
P. S. Tsai, S. Backus, C. G. Durfee, D. Kleinfeld and J. A. Squier, Temporally focused femtosecond laser pulses for low
         
numerical aperture micromachining through optically transparent materials
. Optics Express 18:18086-18094, (2010).

14.  B. Chen, B. Friedman, Q. Cheng,
P. S. Tsai, E. Schim, D. Kleinfeld, and P. D. Lyden, Severe blood-brain barrier disruption and surronding tissue injury, Stroke,
         
40
, E666-E674 (2009).

13.  P. S. Tsai
, J. Kaufhold, P. Blinder, B. Friedman, P.J. Drew, H.J. Karten, P. D. Lyden and D. Kleinfeld, Correlations of neuronal and microvascular densisties in
         
murine cortex revealed by direct counting and colocalization of nuclei and vessels,
Journal of Neuroscience, 29(46) 14553-14570 (2009).

12.  P. S. Tsai
, P. Blinder, B. J. Migliori, J. Neev, Y. Jin, J. A. Squier and D. Kleinfeld, Plasma-mediated ablation: An optical tool for submicrometer surgery on
         
neuronal and vascular systems.
Current Opinion in Biotechnology, 20(1), 90-99 (2009).

11.  B. Friedman, C. Schachtrup,
P. S. Tsai, A. Y. Shih, J. Nordberg, K. Akassoglou, D. Kleinfeld and P. D. Lyden, Acute vascular disruption and Aquaporin 4 loss
         
after stroke,
Stroke, 40(6), 2182-2190, (2009).

10.  A. Shih, B. Friedman, P. Drew,
P. S. Tsai, P. Lyden, and D.Kleinfeld, Active dilation of penetrating arterioles restores red blood cell flux to penumbral neocortex
         
after focal stroke,
Journal of Cerebral Blood Flow and Metabolism, 29, 738-751. (2009).

9.    P. S. Tsai
, B. Migliori, K. Campbell, T. N. Kim, Z. Kam, A. Groisman, and D. Kleinfeld, Spherical aberration correction in nonlinear microscopy and optical
         
ablation using a transparent deformable membrane,
Applied Physics Letters, 91(19), 191102 (2007).

8.    Q. T. Nguyen,
P. S. Tsai, D. Kleinfeld, MPScope: A versatile software suite for multiphoton microscopy, Journal of Neuroscience Methods, 156 (1-2), 351-359
         
(2006).


7.    G. O. Clay, A. C. Millard, C. B. Schaffer, J. Aus-der-Au,
P. S. Tsai, J. A. Squier, and D. Kleinfeld, Spectroscopy of third harmonic generation: Evidence for
          resonances in model compounds and ligated hemoglobin
, Journal of the Optical Society of America B, 23 932 (2006).

6.    N. Nishimura, C. B. Schaffer, B. Friedman,
P. S. Tsai, P. D. Lyden, and D. Kleinfeld, Targeted insult to individual subsurface cortical blood vessels using
          ultrashort laser pulses: Three models of stroke
. Nature Methods, 3, 99 (2006).

5.    C. B. Schaffer, B. Friedman, N. Nishimura, L. F. Schroeder,
P. S. Tsai, F. F. Ebner, P. D. Lyden, and D. Kleinfeld, Two-photon imaging of cortical surface
          microvessels reveals a robust redistribution in blood flow after vascular occlusion,
Public Library of Science Biology, 4 (2), e22 (2006).

4.    P. S. Tsai
, B. Friedman, A. I. Ifarraguerri, B. D. Thompson, V. Lev-Ram, C. B. Schaffer, Q. Xiong, R. Y. Tsien, J. A. Squier, and D. Kleinfeld, All-Optical
          Histology Using Ultrashort Laser Pulses
, Neuron, 39, 27 (2003).

3.    J. A. Davis,
P. S. Tsai, D.M. Cottrell, T. Sonehara, J. Amako, Transmission Variations in Liquid Crystal Spatial Light Modulators Caused by Interference and   
          Diffraction Effects.
Optical Engineering, 38, 1051 (1999).

2.    J. A. Davis,
P. S. Tsai, K.G. D’Neilly, Simple Technique for Determining the Extraordinary Axis Direction for Twisted-Nematic Liquid Crystal Spatial Light
          Modulators
, Optical Engineering, 38, 929 (1999).

1.    J. A. Davis, I. Moreno,
P. S. Tsai, Polarization Eigenstates for Twisted-Nematic Liquid-Crystal Displays, Applied Optics, 37, 937 (1998)





BOOK CHAPTERS

P. S. Tsai
, P. Blinder, J. A. Squier and D. Kleinfeld. All-optical, in situ histology of brain tissue with femtosecond laser pulses, in Imaging in Neuroscience and Development, R. Yuste, series editor. Book 2: Optical Imaging in Neuroscience, F. Helmchen and A. Konnerth, editors, 2010, Cold Spring Harbor Laboratory Press, NY, Chapter 99, in press

P.S. Tsai, and D. Kleinfeld. In vivo two-photon laser scanning microscopy with concurrent plasma-mediated ablation: Principles and hardware realization in Methods for In Vivo Optical Imaging, Second Edition, R. D. Frostig, editor, CRC Press, 3:59-115 (2009)

A. Devor, A. Y. Shih, P. S. Tsai, P. Blinder, P. Tian, and D. Kleinfeld. Two-photon laser scanning microscopy as a tool to study cortical vasodynamics under normal and ischemic condition. in Imaging the Brain with Optical Methods, A. Roe, editor, Springer Press, 11:245-262 (2009)

Q. -T. Nguyen, G. O. Clay, N. Nishimura, C. B. Schaffer, L. F. Schroeder, P. S. Tsai, and D. Kleinfeld, Pioneering applications of two-photon microscopy to mammalian neurophysiology: Seven case studies in Handbook of Biomedical Nonlinear Optical Microscopy, B. Masters and P. So, editors. Oxford Press, pp 715-734 (2008)

P. S. Tsai
, B. Friedman, C. B. Schaffer, J. A. Squier and D. Kleinfeld, All-optical, in situ histology of neuronal tissue with femtosecond laser pulses in Imaging in Neuroscience and Development: A Laboratory Manual , R. Yuste and A. Konnerth, ed., Cold Spring Harbor Laboratory Press, NY pp 439-444 (2005)

P. S. Tsai
, N. Nishimura, E. J. Yoder, E. M. Dolnick, G. A. White, and D. Kleinfeld, Principles, design, and construction of a two photon laser scanning microscope for in vitro and in vivo brain imaging in In Vivo Optical Imaging of Brain Function, R. D. Frostig, ed.,Broca Raton, CRC Press, pp. p113-171 (2002).



PATENTS

D. Jeong, D. Kleinfeld, J. Neev, and
P. S. Tsai, Device and method for selective removal of bone versus soft tissue using ultrashort laser pulses and spectroscopic feedback. Provisional patent submitted December, 2009

B. Friedman, D. Kleinfeld, P. D. Lyden, N. Nishimura, C. B. Schaffer, L. F. Schroeder, and
P. S. Tsai, Device and method for inducing vascular injury and/or blockage in an animal model, United States Patent no. 7,258,687.



TRADE JOURNALS


N. Nishimura, C. B. Schaffer, B. Friedman,
P. S. Tsai, P. D. Lyden and D. Kleinfeld, Laser photoablation: A new biomedical tool, SPIE Newsroom, http://spie.org/x8614.xml?highlight=x2416 (2006)

G. O. Clay, N. Nishimura, C. B. Schaffer,
P. S. Tsai, and D. Kleinfeld, Beyond observation: microscopy with ultrashort laser pulses to probe and manipulate cortical vasculature, SPIE Optics in Information Systems 15, 4 (July 2004).

P. S. Tsai
, J. A. Squier, and D. Kleinfeld, Ultra-short laser light: a cool tool for ultra-precise tissue cutting, Optics and Photonics News 15, 7 (July 2004) pp 24-29.



CONFERENCE PROCEEDINGS
 
J. Kaufhold, P. S. Tsai, P. Blinder and D. Kleinfeld. Threshold relaxation is an effective means to connect gaps in 3D images of complex microvascular networks, in Proceedings of the 3rd Workshop on Microscopic Image Analysis with Applications in Biology, in conjunction with MICCAI, (2008)

N. Nishimura, C. B. Schaffer, B. Friedman,
P. S. Tsai, P. D. Lyden, and D. Kleinfeld, Targeted disruption of deep-lying neocortical microvessels in rat using ultrashort laser pulses, Proceedings of SPIE Vol. 5340 Commercial and Biomedical Applications of Ultrafast Lasers IV, (2004) p.179-187.

A. Ifarraguerri, B. Thompson,
P. S. Tsai, B. Friedman, C. B. Schaffer, V. Lev-Ram, R. Y. Tsien , J. A. Squier, and D. Kleinfeld, Quantitative analysis of volumetric TPLSM data, in Proceedings of IEEE International Symposium on Biomedical Imaging(2004) p.804-807 vol. 1

C. B. Schaffer, N. Nishimura, B. Friedman,
P. S. Tsai, L. F. Schroeder, F. F. Ebner, P. D. Lyden, and D. Kleinfeld, Two-photon microscopy reveals that cortical blood flow reverses direction at the first branch that lies downstream from a localized photothrombotic clot, in Technical Digest: OSA Conference on Lasers and Electro-Optics (2004)

P. S. Tsai
, B. Friedman, A. I. Ifarraguerri, B. D. Thompson, V. Lev-Ram, C. B. Schaffer, Q. Xiong, R. Y Tsien, J. A. Squier, and D. Kleinfeld, All optical histology: serial ablation and multiphoton imaging of neuronal tissue with femtosecond laser pulses, in Proceedings of IEEE Lasers and Electro-Optics Society, (2003). p.485-486 vol.2

C. B. Schaffer, F. F. Ebner, N. Nishimura, B. Friedman,
P. S. Tsai, L. F. Schroeder, P. D. Lyden, and D. Kleinfeld, Arteriole blood flow reverses direction at the first branch that lies downstream from a localized photothrombotic clot, Proceedings of IEEE Lasers and Electro Optics Society Annual Meeting (2003) p.307.

C. B. Schaffer, F. F. Ebner, N. Nishimura, B. Friedman,
P. S. Tsai, L. F. Schroeder, P. D. Lyden, and D. Kleinfeld, Two-photon fluorescence microscopy of collateral blood flow following photothrombotic stroke in rat neocortex, in Technical Digest: OSA Annual Meeting (2003).

C. B. Schaffer, F. F. Ebner, N. Nishimura, B. Friedman,
P. S. Tsai, L. F. Schroeder, P. D. Lyden, and D. Kleinfeld, Real-time two-photon fluorescence microscopy of blood flow dynamics following photothrombotic stroke in rat neocortex, in Lasers and Electro-Optics Society, (2003). p.307-308 vol. 1

P. S. Tsai
, B. Friedman, V. Lev-Ram, Q. Xiong, R. Y. Tsien, D. Kleinfeld, A. I. Ifarraguerri, B. D. Thompson and J. A. Squier, All optical histology of brain tissue: Serial ablation and multiphoton imaging with femtosecond laser pulses in Proceedings of Conference on Lasers and Electro-Optics, Baltimore, MD (2003). p 4-7

C. B. Schaffer, L.F. Schroeder, P. D. Lyden, N. Nishimura,
P. S. Tsai, and D. Kleinfeld All-optical thrombotic stroke model for near-surface blood vessels in rat: focal illumination of exogeneous photosensitizers combined with real-time two-photon imaging in Proceedings of SPIE Vol. 4978 (2003), p.222-231.



INVITED PRESENTATIONS

P. S. Tsai
, QBio : An Integrative Educational Program of Physics and Biology at UCSD.  AAPT Winter Meeting (Jan, 2018)

P. S. Tsai, Guiding the Transition: Biophysics Laboratory on Research Design and Implementation.  AAPT Winter Meeting (Jan, 2015).

P. S. Tsai, Optical tools for high-resolution, large-field measurements in the brain.  Optics and photonics research at UCSD: materials, metamaterials, living and synthetic matter (Aug, 2014).

P. S. Tsai, Mouse cerebral blood flow and reconstruction of the cortical angioarchitecture. VRC Seminar at Katholieke Universiteit Leuven  (Apr, 2014).

P. S. Tsai, Blood flow and the angioarchitecture of mouse cerebral cortex . Neuronus 2014 (Apr, 2014).

P. S. Tsai, Bloodflow and the angio-architecture in relation to the vibrissa barrels. Barrels XXVI (Nov, 2013).

P. S. Tsai, D. C. Jeong, D. Kleinfeld,  Precise Cranial Surgery With Femtosecond Laser Ablation, Laser Induced Breakdown Spectroscopy and Second Harmonic Generation.  SCIX / North American Society for Laser Induced Breakdown Spectroscopy (Oct, 2013).

P. S. Tsai
, P. Blinder, J. P. Kaufhold, and D. Kleinfeld, From Photons to Flow Patterns – Quantifying neurovascular architecture with light microscopy. Janelia Farms Conference on Multiphoton Imaging: The Next 6x10^23 Femtoseconds (April, 2011)

P. Blinder,
P. S. Tsai, J.P. Kaufhold, and D. Kleinfeld, Reconstruction of the cortical vascular network in mouse, Computational Neurosciences Meeting, Workshop on High-throughput 3D microscopy and high-performance computing for multi-scale modeling and simulation of large-scale neuronal circuits (July, 2010)

P. S. Tsai,
P. Blinder, P. Drew, J. Driscoll, D. Jeong, J.P. Kaufhold, A. Shih, I. Valmianski, and D. Kleinfeld, Topology, dynamics, and control in cortical blood flow elucidated with optical techniques, SPIE Optics and Photonics (August, 2009)

P. S. Tsai
, P. Blinder, B. Migliori, D. Kleinfeld, Ultra-Short Laser Pulses as a Tool to Measure as well as Perturb Neurovascular Activity in the Rodent Brain, Optical Society of America, Annual Meeting: Frontier in Optics/ Laser Science (October, 2008)

P. S. Tsai,
Challenges in the analysis and acquisition of 3-D in vivo imaging data, Fourth International Workshop Statistical Analysis of Neuronal Data (May, 2008)

P. S. Tsai
, B. Friedman, A. I. Ifarraguerri, B. D. Thompson, V. Lev-Ram, C. B. Schaffer, Q. Xiong, R. Y. Tsien, J. A. Squier, and D. Kleinfeld, All optical histology: Serial ablation and Multiphoton Imaging of Neuronal Tissue with Femtosecond Laser Pulses, IEEE Lasers and Electro Optics Society Annual Meeting (October, 2003)

P. S. Tsai
, B. Friedman, V. Lev-Ram, Q. Xiong, R. Y. Tsien, D. Kleinfeld, A. I. Ifarraguerri, B. D. Thompson, and J. A. Squier*, All optical histology of brain tissue: Serial ablation and multiphoton imaging with femtosecond laser pulses, Conference on Lasers and Electro-Optics (May, 2003)



CONTRIBUTED PRESENTATIONS

P. Blinder, P. S. Tsai, J. P. Kaufhold, P. M. Knutsen, H. Suhl and D. Kleinfeld, Highly interconnected microvasculature supports restricted blood flow independent of columnar boundaries, 21st Annual Meeting of the Israel-Society-for-Neuroscience (Nov, 2013).

P. S. Tsai, M. Anderson,  J. Field, C. Mateo, and D. Kleinfeld, Ultrawide-field two photon laser scanning microscope, Society for Neuroscience Annual Meeting (November, 2013).

C. Mateo, P. S. Tsai, A. Y. Shih, and D. Kleinfeld,  Probing neurovascular coupling via natural and driven vasomotion in the awake mouse, Society for Neuroscience Annual Meeting (November, 2013).

P. S. Tsai
, P. Blinder, J. Kaufhold, B. Friedman, P. D. Lyden, and D. Kleinfeld, The murine angiotome: Patterns of blood flow in mouse vibrissa sensory cortex revealed by modeling flow in vectorized reconstructions of the vasculature, Society for Neuroscience Annual Meeting (November, 2011)

A. Y. Shih, P. Blinder,
P. S. Tsai, G. M. Stanley, B. Friedman, P. D. Lyden, and D. Kleinfeld, The smallest stroke: Occlusion of a single penetrating vessel in cortex leads to local pathology and sensory impairment, Society for Neuroscience Annual Meeting (November, 2011)

P. Blinder,
P. S. Tsai, J. P. Kaufhold, and D. Kleinfeld, Topological basis for differences in surface versus subsurface blood flow from the vectorization of all vessels and cell nuclei across cortical columns, Society for Neuroscience Annual Meeting (November, 2010)

P .S. Tsai
, P. J. Drew, A. Y. Shih, P. Knutsen, D. Davalos, P. Blinder, K. Akassaglou, and D. Kleinfeld, Large-scale chronic imaging and optical manipulation of cortical cells through a polished and reinforced thinned-skull, Society for Neuroscience Annual Meeting (November, 2010)

D. Jeong,
P. S. Tsai, D. Schafer, J. Squier, Y. Neev, and D. Kleinfeld, All optical cranial surgery: Ablation of hard but not soft tissue by amplified femtosecond laser pulses and feedback from atomic emission spectra, Society for Neuroscience Annual Meeting (November, 2010)

P. S. Tsai
, P. Blinder, J. P. Kaufhold, B. Friedman, P. D. Lyden, and D. Kleinfeld, Vascular and cellular maps of mouse cortex revealed through all-optical histology and volumetric image data analysis algorithms, Society for Neuroscience Annual Meeting (November, 2009)

P. J. Drew, J. D. Driscoll,
P. S. Tsai, A. Y. Shih, and D. Kleinfeld, Two-photon imaging of spontaneous and sensory evoked cortical microvascular dynamics in the awake mouse, Society for Neuroscience Annual Meeting (November, 2009)

J. Kaufhold,
P. S. Tsai, P. Blinder and D. Kleinfeld. Threshold relaxation is an effective means to connect gaps in 3D images of complex microvascular networks, Microscopic Image Analysis with Applications in Biology / Medical Image Computing and Computer Assisted Intervention (September, 2008)

P. Blinder,
P. S. Tsai, J. Kaufhold, A. Y. Shih, C. A. Rafie, B. Friedman, P. D. Lyden, and D. Kleinfeld, Towards a multi-scale complete mapping of the cortical rodent angioarchitecture and neuronal cell nuclei distribution: a potential structural basis for post-ischemic cell damage patterm. Gordon Conference on Brain Energy Metabolism & Blood Flow (August 2008),

P. S. Tsai
, J. Kaufhold, B. Friedman, P. Lyden, D. Kleinfeld , Direct Counts of Cellular and Vascular Densities in Thick Cortical Sections of Mouse. , Society for Neuroscience Annual Meeting (November, 2007).

J. Kaufhold,
P. S. Tsai, P. Binder, B. Friedman, P. Lyden, D. Kleinfeld, A. I. Ifarraguerri , Automatic Neuroanatomical Microvessel Segmentation, Nuclei Labeling and Diffusion Distance Computations with All Optical Histology, Society for Neuroscience Annual Meeting (November, 2007).

P. Binder,
P. S. Tsai, J. Kaufhold, B. Friedman, D. Kleinfeld, Complete Millimeter-Scale Reconstruction of Cells and Vasculature in Mouse Barrel Cortex , Society for Neuroscience Annual Meeting (November, 2007).

B. Migliori,
P. S. Tsai, K. Campbell, T. Kim, T. Kam, A. Groisman, D. Kleinfeld , A Flexible Polymer Lens Reduces Spherical Aberration in Two-Photon Microscopy, Society for Neuroscience Annual Meeting (November, 2007).

P. S. Tsai
, T.N. Kim, K. Campbell, A. Groisman, Z. Kam, and D. Kleinfeld, Spherical aberration correction in deep multi-photon imaging, Optical Society of America, FiO (2005)

N. Nishimura, C. B. Schaffer, B. Friedman,
P. S. Tsai, P. D. Lyden, and D. Kleinfeld, Collateral blood flow after occlusion of individual surface and subsurface microvessels, Barrels XVII (2004).

P. S. Tsai
, B. Friedman, A. I. Ifarraguerri, C. B. Schaffer, V. Lev-Ram, R. Y. Tsien, and D. Kleinfeld, All-optical histology for the large-scale mapping of cellular and vasculature distributions in rodent brain, Focus on Microscopy Conference (April 2004).

N. Nishimura, C. B. Schaffer, B. Friedman,
P. S. Tsai, P. D. Lyden, and D. Kleinfeld, Targeted microvascular disruption in rat cortex using ultrashort laser pulses, Focus on Microscopy (2004).

N. Nishimura, C. B. Schaffer, B. Friedman,
P. S. Tsai*, P. D. Lyden, and D. Kleinfeld, Targeted disruption of deep-lying neocortical microvessels in rat using ultrashort laser pulses, Photonics West (2004).

A. Ifarraguerri, B. Thompson,
P. S. Tsai, B. Friedman, C. B. Schaffer, V. Lev-Ram, R. Y. Tsien , J. A. Squier, and D. Kleinfeld, Quantitative analysis of volumetric TPLSM data, IEEE International Symposium on Biomedical Imaging (2004)

C. B. Schaffer, N. Nishimura, B. Friedman,
P. S. Tsai, L. F. Schroeder, F. F. Ebner, P. D. Lyden, and D. Kleinfeld, Two-photon microscopy reveals that cortical blood flow reverses direction at the first branch that lies downstream from a localized photothrombotic clot, OSA Conference on Lasers and Electro-Optics (May, 2004)

P. S. Tsai
, B. Friedman, A. I. Ifarraguerri, B. D. Thompson, V. Lev-Ram, C. B. Schaffer, Q. Xiong, R. Y. Tsien, J. A. Squier, and D. Kleinfeld , All optical histology of neuronal tissue using ultrashort pulses, Society for Neuroscience Annual Meeting (November, 2003).

N. Nishimura, C. B. Schaffer,
P. S. Tsai, B. Friedman, P. D. Lyden, and D. Kleinfeld, Photodisruption with amplified femtosecond laser pulses induces three-dimensionally localized clots in neocortical vasculature in rat, Society for Neuroscience Annual Meeting (November, 2003)

C. B. Schaffer, F. F. Ebner, N. Nishimura, B. Friedman,
P. S. Tsai, L. F. Schroeder, P. D. Lyden, and D. Kleinfeld, Two-photon fluorescence microscopy of collateral blood flow following photothrombotic stroke in rat neocortex, OSA Annual Meeting (Oct, 2003).

C. B. Schaffer, F. F. Ebner, N. Nishimura, B. Friedman,
P. S. Tsai, L. F. Schroeder, P. D. Lyden, and D. Kleinfeld, Arteriole blood flow reverses direction at the first branch that lies downstream from a localized photothrombotic clot, Lasers and Electro Optics Society Annual Meeting (Oct, 2003)

P. S. Tsai
, B. Friedman, V. Lev-Ram, Q. Xiong, R. Y. Tsien, D. Kleinfeld, A. I. Ifarraguerri, B. D. Thompson, and J. A. Squier*, All optical histology of brain tissue: Serial ablation and multiphoton imaging with femtosecond laser pulses, Conference on Lasers and Electro-Optics (May, 2003).

C. B. Schaffer, F. F. Ebner, N. Nishimura, B. Friedman,
P. S. Tsai, L. F. Schroeder, P. D. Lyden, and D. Kleinfeld, Real-time two-photon fluorescence microscopy of blood flow dynamics following photothrombotic stroke in rat neocortex, Lasers and Electro-Optics Society, (May, 2003).

P. S. Tsai
, B. Friedman, A. I. Ifarraguerri, B. D. Thompson, V. Lev-Ram, C. B. Schaffer, Q. Xiong, R. Y. Tsien, J. A. Squier, and D. Kleinfeld, All Optical histology of neuronal tissue : serial ablation and multiphoton imaging with femtosecond laser pulses, SPIE Photonics West (January 2003).

C. B. Schaffer, L. F. Schroeder, P. D. Lyden, N. Nishimura*,
P. S. Tsai*, and D. Kleinfeld, Femtosencond laser-induced hemorrhagic and thrombolytic microstrokes in rodents, SPIE Photonics West (January 2003).

P. S. Tsai
, J. A. Squier, and D. Kleinfeld, Precision, Large-Scale Cutting Of Neuronal Tissue with Femtosecond Laser Light, Biophysical Society Annual Meeting (Feburary 2002).

O. Clay,
P. S. Tsai*, A. C. Millard, J. A. Squier, and D. Kleinfeld, Evidence for Resonances in the Third Harmonic Spectra of Hemoglobin as a Function of its Ligand Binding State, Biophysical Society Annual Meeting (Feburary, 2002).

O. Griesbeck, E. J. Yoder*,
P. S. Tsai*, R. Y. Tsien, and D. Kleinfeld, In-Vivo Expression of Cameleons in Rodent Neocortex by Adenoviral Gene Transfer, Society for Neuroscience Annual Meeting (November, 2000).